The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items had been digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A point mutation was released by the fast modify https://bzatp-triethylammonium-sa33210.estate-blog.com/26308280/everything-about-hymenidin